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Sea lions may even prove useful for testing to treat or prevent epilepsy, he says: "I'm impressed by how the investigators contended successfully with the limitations and constraints of experimenting with wild animals".
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The amplicon size is user-defined according to the constraints of the experiment, e.g. accurate sequencing limits amplicons to a few hundred base pairs.
Although these postscan memory assays were probably more susceptible to false memories and distortions than if they had been presented immediately following each song in the scanner, I felt they were a reasonable compromise given the technical constraints of the experiment.
Beginning in the late 1950s, however, there were a variety of poets and schools who rebelled against these constraints and experimented with more-open forms and more-colloquial styles.
Additionally, this study attempts the LES for estimation of the wind effects on the prototype stadium with retractable roof, which can account for the Reynolds number effects and overcome the model space constraints of wind tunnel experiments.
Within the constraints of these limitations, experiments in this paper suggest an intriguing possibility.
The main constraint of our experiment is simplifications in the model.
Some of the deficiencies of experimental data sets are (i) an insufficient number of time-points, (ii) a very large experimental error at each time point, due to the experimental difficulties as well as a small sample size, and (iii) the long-term dynamics is often not captured due to the time-constraints of the experiment.
Another constraint of these experiments was the significant run down of the [Ca2+]i elevations upon repetitive 3,5-DHPG applications (to 40±16% of the initial amplitudes, two applications, n = 4), probably due to wash-out of cytosolic constituents during whole cell recording.
A second potential concern involves the constraints of the time course experiment itself.
The aim was to find the most efficient combination of materials under the constraints of an extreme low-background experiment operating in a typical underground laboratory.
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