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One constraint of our study is that a limited number of patients received cetuximab (EGFR antagonist) as a bio-chemotherapy throughout the entire course of treatment.
Firstly, data were collected from adolescents enrolled in schools (the secondary education enrollment rate is 35% in Pakistan [ 21]) therefore results could not be generalized on youngsters not attending school that remains a major constraint of our study.
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There were constraints of our study.
27 Our results should be interpreted within the power constraints of our study.
The STROBE statement (strengthening the reporting of observational studies in epidemiology) was followed, within the constraints of our study design.
Other constraints of our study are its retrospective nature and the young age distribution of the study cohort, with a median age of 13 years.
However, within the power constraints of our study, age-adjusted mean TRF in WBCs is not a marker of breast cancer susceptibility.
Single-point approaches to the meta-analysis of low-frequency variants performed poorly with respect to false-positive rate and, within the power constraints of our study, almost never detected all causal alleles.
To exclude the possibility that our null results for autosomal variants are due to either sampling bias or data quality and to examine the probability of having false positives within the power constraints of our study, we sought a theoretical corroboration of our empirical results by conducting association analysis in an 'ideal' unbiased simulated dataset.
Future extensions of the approach pioneered here could test more thoroughly how condition-specific remote TMS effects upon the visual cortex may vary for different levels of the cortical hierarchy, including use of more extensive mapping of visual areas than was possible here given the time constraints and scope of our study.
Still, the limitations of our study include constraints in the gene annotation in the selected linkage regions and the availability of raw genome-wide data.
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