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2) The authors use 97% sequence identity threshold for EST alignments (it seems without constraining alignment length).
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The sum of deviation scores in consecutively constrained alignment columns provides a metric of evolutionary constraint, termed a rejected substitution (RS) score.
▪ The constrained alignment problem where and is NP-complete.
P roposition 2.1 The constrained alignment problem where and is NP-complete.
The maximum weight independent set (MWIS) of provides an optimum solution to the constrained alignment problem.
A neighborhood around each anchor can be added to increase the flexibility of the constrained alignment.
First of all we provide a novel constrained alignment framework appropriate for the one-to-many alignments model.
We first state that the constrained alignment problem defined herein is computationally intractable even in a restricted case.
We show that the constrained alignment problem even in a primitive setting is computationally intractable, which justifies efforts for designing efficient heuristics.
The constrained alignment problem where and k2 any positive integer constant is polynomially solvable if one of the directed graphs Gp or is acyclic.
P roposition 2.2 The constrained alignment problem where and k2 any positive integer constant is polynomially solvable if one of the directed graphs Gp or is acyclic.
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