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Using a linear model, we distinguish between constitutive, alternative and alternatively regulated polyadenylation sites.
The data presented is strongly indicative of a constitutive, alternative pathway, which resulted in increased levels of basal NOS activity.
In the current study we demonstrate robust NO production by astroglia in the mutants' neocortex, suggestive of a constitutive alternative pathway.
This observation led to generation of transgenic mice expressing a stabilized form of NIK, using a tissue-specific Cre-mediated activation approach, which results in tissue-specific constitutive alternative pathway activation [15].
Probes included probes for constitutive, alternative exonic; alternative spliced/skipped exons, mitochondrial genes (mtDNA) for more details on oligos, we refer to [ 83].
An exon can be either completely constitutive, or completely alternative, or non-coding, or consist of constitutive, alternative and non-coding regions.
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A study of constitutive versus alternative splicing, has examined the SYT7 alternative exons [ 75].
For introns shorter than 1.5 kb, constitutive and alternative introns displayed opposite, slight (constitutive R = -0.053, alternative R = 0.026) but statistically significant (constitutive P = 3.34e-34, alternative P = 6.27e-5) correlations between intron length and splice site strength.
Sequencing and assembly of the micronuclear genomes of more of these ciliate species will provide future insights into the structural arrangement of constitutive and alternative MDSs in the germline and the mechanisms that drive alternative DNA processing.
We found that, for human introns greater than 1.5 kb in length [the median length of human introns;], total splice site strength is positively correlated with intron length for both constitutive and alternative introns; the correlation coefficients were relatively small (constitutive R = 0.124, alternative R = 0.145) but highly statistically significant (P ≈ 0).
Since the microarrays included constitutive and alternative splice variant probes, we examined how many genes were alternatively spliced in response to HAL-PDT (Table 3).
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