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High concentration of fluoride, often above 1.5 mg L−1, constitutes a severe problem over a large part of India.
Contaminating human DNA constitutes a severe problem since it may be indistinguishable from the endogenic DNA (especially if the donors are ethnically related to the subjects), and exogenic DNA may, after relatively few years, accumulate damage patterns that are similar to those of the endogenic DNA [39].
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However, because we compared relative differences across the experimental groups, this limitation should not constitute a severe problem for our analyses.
These genes constitute a severe problem because primers could hybridize in a large number of genomic places, giving rise to multiple PCR products.
Malnutrition constitutes a serious problem of public health.
This problem affects all colocalization analysis and thus constitutes a serious problem of the study.
Septic shock is a major therapeutic problem in intensive care units (ICUs) and constitutes a severe hemodynamic and immune challenge for the patient.
Quarry dust and marble sludge, produced in large quantities from quarries, aggregates and ornamental stone plants, constitute a severe environmental problem.
Defects in tissue repair constitute a severe health problem and are frequently seen not only in older individuals but in patients with diabetes, those receiving chemo- or radiotherapy, and other patients whose immune system is suppressed (reviewed in [ 38]).
Yet software piracy continues to be a severe problem.
3 Unfortunately, knee injuries are common, and severe injuries such as anterior cruciate ligament injury constitute a serious problem in terms of long absence from or termination of football and early osteoarthritis.
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