In the foot, 5 of the top 10 most abundant annotated transcripts were homologues of foot proteins identified from M. californianus and M. galloprovincialis datasets, specifically adhesive plaque matrix proteins, which constituted 477 reads (1.5% of total).
For the purpose of analysis, the highest reading constituted the PEFR reading for the patient.
For journal articles from category two, which constituted core reading and were from journals for which our institution had paid a subscription, we requested permission to store an electronic version (Pdf) on a secure password protected server to which only course students and staff had access.
Using this information on normal globin levels in pig blood, we estimate the globin depletion protocol to have worked well for 16 of the 32 samples used here where the total globin reads constituted below 15% of the total mapped reads.
A fluorescence reading (excitation: 545 nm, emission: 590 nm) was taken using a Perkin Elmer LS-50 fluorimeter; this constituted the baseline reading.
Single copy reads constituted 85.3%, 85.6%, and 84.7% of unique reads in flowers, leaves, and PRSV infected leaves, while reads with over 10 copies constituted only 1.4%, 1.2%, and 2.3% of the unique reads.
The total number of uniquely mapped reads constituted a gene's transcript abundance.
In sum, the clean reads constituted ~38 GB of sequence data.
All best-scoring alignments were reported and indels with a minimum support of two reads constituted the indel candidate set to be further evaluated.
