Temperature cycling consisted of 72°C for 5 min, 98°C for 30 s followed by 18 cycles of 98°C for 30 s, 65°C for 30 s, 72°C for 30 s with a final Taq extension step at 72°C for 5 min. These amplified sample pools constitute a sequencing "library".
Temperature cycling consisted of 72° for 5 min, 98° for 30 s, followed by 18 cycles of 98° for 30 s, 65° for 30 s, and 72° for 30 s, with a final Taq extension step at 72° for 5 min. These amplified sample pools constitute a sequencing "library".
That hardly constitutes a sequence, however, and the obvious suspicion is that this was Mourinho's point.
Early human brain development constitutes a sequence of intricate processes resulting in the ontogeny of functionally operative neural circuits.
The corruption charges constituted a sequence in the power struggle that first erupted in 2011 with a massive match fixing scandal, the worst in Turkish soccer history, in which the two Islamist leaders battled for control of storied Istanbul club, Fenerbahce SK, the political crown jewel in Turkish soccer.
Their products are processed from a 45S precursor rRNA, which begins with a 5'-external transcribed sequence; the first 414 nucleotides of this constitute a leader sequence and is the first part to be removed [6].
Furthermore, thus far no attention has been paid to the possible sequence variations in the seven plasmids, which constitute a total sequence length of 383 486 bp almost 10% of the total coding capacity of Synechocystis sp. PCC 6803.
These, which can be diagrammed as a linear sequence (though often embedded within larger branching networks), constitute a large sequence of steps, one that eventuates in morphological change.
