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The acid-soluble constituents were separated from the dark-colored precipitates by concentrated urea treatment and accounted for 16 45 % of the total organic carbon in HAs.
Our previous study has demonstrated that acid-soluble constituents were separated from HAs during preparative PAGE in the presence of concentrated urea [7].
The chemical constituents were separated using any one of the three different eluent systems.
These constituents were separated away on the basis of polarity of different solvents in to different fractions.
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Forewings were combusted, and constituent gases were separated on a gas chromatograph column linked to a mass spectrometer.
Furthermore, soil HA constituents with different fluorescent properties were separated by ultrafiltration in the presence of concentrated urea [11, 12].
The constituents of the volatile bouquet were separated with a DB-5MS column (Agilent, Santa Clara, CA, USA, 30 m × 0.25 mm × 0.25 μm) and He (MS) or H2 (FID) as carrier gas.
The constituents of the volatile bouquet were separated using a ZB-WAX column (Phenomenex, Aschaffenburg, Germany, 60 m × 0.25 mm × 0.15 μm) and He (MS) or H2 (FID) as carrier gas.
Multiple infections (3.7% of all ICC cases) were separated into constituent types, thus type-specific prevalence represents that in either single or multiple infections.
Our previous study on two-dimensional (2-D) electrophoresis of HAs in the presence of 7 M urea showed that the HAs were separated into their constituents with different charge characteristics and molecular sizes [4].
Chemical constituents of the extracts (100 μg) were separated using aluminium-backed TLC plates (Merck, silica gel 60 F254).
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