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Initially the extracts or purified constituents were dissolved in DMSO and the dilutions of samples tested were made in the respective buffer for each enzyme, i.e., 0.1 M sodium phosphate (pH 7.5) for subtilisin and 0.1 M sodium acetate (pH 4.4) for pepsin.
Boiogito and the extracts of its six constituents were dissolved in a solution of 5%% dimethylsulfoxide in PBS and centrifuged for 5 min at 10,000 rpm.
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Key chemical constituents are dissolved gases, dissolved inorganic ions and compounds, particulate inorganic material, particulate organic material, and dissolved organic ions (nitrogen and phosphorus) and compounds.
Each chemical constituent was dissolved in 25 ml de-ionized water.
For each NH4 +-level, the constituents per liter of medium were dissolved in the corresponding solution to a final volume of 1000 mL.
The constituents of the production medium were dissolved in the buffer solution and poured into the flask contained 50 mL of the buffered medium at the particular pH.
If parliament were dissolved, the constituent assembly drawing up the constitution would be abolished with it.
This would have been the second time the constituent assembly was dissolved because of disputes over the criteria of selecting its members.
All the other necessary constituent materials are dissolved in a solvent which is then spin-coated over a substrate.
However, after four years, no constitution had been written and consequently the constituent assembly was dissolved in 2012.
The declaration also said the constituent assembly could not be dissolved by the judiciary, pre-empting an expected ruling by the Supreme Constitutional Court on its legitimacy.
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