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Acetate supporting buffer (0.02 M in each constituent) was prepared by dissolving 1.68 g of sodium acetate (Winlab, UK) in 500 ml distilled water containing 1.12 ml of acetic acid and diluting to one liter with distilled water.
Finally, carbonate supporting buffer (0.1 M in each constituent) was prepared by dissolving 10.6 g of sodium carbonate (BDH, UK) and 8.4 g of sodium hydrogen carbonate (Winlab, UK) in one liter distilled water.
Britton-Robinson (B-R) supporting buffer (pH 7.5, 0.04 M in each constituent) was prepared by dissolving 2.47 g of boric acid (Winlab, UK) in 500 ml distilled water containing 2.3 ml of glacial acetic acid (BDH, UK) and then adding 2.7 ml of ortho-phosphoric acid (Riedal-deHaen, Germany) and diluting to one liter with distilled water.
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Transparent and highly conducting (10−3 S cm−1) composite gel electrolytes (cges) based on poly methylmethacrylate) (PMMA) with 1 M LiClO4 in propylene carbonate (PC) and hydrophilic fumed silica (SiO2) added in different proportions up to 5 wt.% as the constituents were prepared.
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SbSI xerogel was prepared sonically from the constituents (the elements: antimony, sulfur, and iodine).
The product was prepared using the identical constituents obtained from the same commercial source as the components of the branded product NYDA®.
A SCAmpPs cyclic peptide-enriched fraction was prepared by fractionating the methanol-soluble constituents from the phloem powder with ethyl acetate and ethanol and resuspended in water [ 35].
KCD was prepared after proper identification and quantification of the constituents under aseptic conditions as per the method described [ 16, 17].
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