Plackett – Burman and Central composite design was employed and the optimal medium constituents for maximum cellulase production (4040.45 U/mL) were determined as rice bran, yeast extract, MgSO4·7H2O and KH2PO4 at 6.27, 2.52, 0.57 and 0.39 g/L, respectively.
The optimal combinations of media constituents for maximum α-amylase production were determined as 17.58 g/l starch, 12.37% (v/v) glycerin, 8.77 g/l peptone and 0.00 g/l YE.
The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0.
Using this methodology, the quadratic regression model of producing cold-active protease was built and the optimal combinations of media constituents for maximum protease production (183.21 U/mL) were determined as casein 5.18 g/L, citrate sodium 3.84 g/L, temperature 7.96 °C, Tween-80 0.23 g/L.
Simulated uptake in smokers of mainstream smoke constituents at maximum sugar application level and from marketed American-blend and Virginia-type cigarettes 267 7.4.
Broad band is composed by at least three constituents with maxima at about 335, 390 400, and 470 nm.
Among them, gentiopicroside (3), one of the main active constituents, was the maximum amount among all the components in both "Long-Dan" and "Qin-Jiao".
The optimal combination of media constituents for the maximum production of bio-indigo was determined as tryptophan 2.4 g l−1, yeast extract 4.5 g l−1 and sodium chloride 11.4 g l−1.
Sharma et al. [ 21] reported that the antioxidant potential of amla may be due to the presence of the many phyto-constituents, which provide maximum conjugation with free radical species, thus reducing the number of free radicals available and the extent of cellular damage.
No significant difference was found between the two groups in the constituent ratio of the maximum temperature (P = 0.064) (Table 5).
This indicated that the relatively smaller HA constituents with an emission maximum at a shorter wavelength were dissociated in the presence of concentrated urea, an observation that was confirmed by the present results.
