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Furthermore, it appears that the artificial induction of constantly high Nanog levels is sufficient to almost completely restrict the ES cell population from undergoing differentiation [11].
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We have stated above that such a network configuration is not able to explain the experimentally observed Nanog fluctuations in the presence of constantly high Oct4/Sox2 levels.
Optimal methane drainage relies on two key factors: constantly high gas flow and high methane concentration.
Whereas the left peak represents the cells that currently express low levels of Nanog, the right peak corresponds to the cells with high Nanog concentrations.
New experimental results provide evidence that the maintenance of pluripotency does not necessarily require continuously high Nanog levels.
These cells are characterized by fluctuations between two clearly different phenotypic states of ES cells; one is stable ("high Nanog"; HN) and the other is unstable ("low Nanog"; LN).
This observation is consistent with high Nanog expression levels in the area of primitive streak formation in the mouse embryo, where Nanog expression co-localized with the primitive streak marker Mixl1 [43], [43].
Multiple Oct4+/− ESC lines have a unimodal high Nanog expression profile and lack Nanog-low undifferentiated cells.
The resistance rate remained constantly high in 2003.
High FGF4 expression is associated with high Nanog expression, whereas high FGF5 and FGF8 expression is characteristic for cells with low levels of Nanog.
ESCs fluctuate between states of high Nanog expression, possessing high self-renewal efficiency and low Nanog expression, exhibiting increased differentiation propensity (Chambers et al., 2007).
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