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We introduced a dynamic parameter, e.g. regulation between two time points, which obviously eliminates genes that are constantly expressed by the endothelium.
Next, we looked at two transgenic lines, D and E, in which GFP is constantly expressed by a ubiquitous class of promoters.
For enumeration of the total CEC number, and not a particular subpopulation, it is necessary to identify markers that are specific for and are constantly expressed by all CEC.
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Another 33.8% of signatures were constantly expressed, defined by Meyers et al. (2004) [ 24] as those signatures with a summed abundance within a two-fold range (expression ratio <2).
These genes were not constantly expressed, as shown by transcriptome data.
Their expression is balanced by the promoters being constantly expressed gearbox promoters.
IL-2R-γ expression in monocytes is known to be induced by the likewise constantly expressed IFN-γ.
However, master regulators of genes in the IRGCs may share a similar expression profile but with a lag time, or they may be constantly expressed throughout SC injury response while being modulated by mechanisms other than transcriptional control.
Higher concentrations within that range are enough to stop the production of the inducer I2 (there is always a small concentration during this time), but are still understood as a logic "0" by our circuit (thus Out is constantly expressed).
Although the TMPRSS2-ERG transcript was significantly and constantly expressed in VCaP cells, which harbor this fusion gene, its expression was induced to a detectable level by the stimulation with DHT in LNCaP cells.
Compared to the study in which they evaluated the heterogeneity of the protein constantly expressed in the certain cellular location, the marker like Ki-67 by its nature do not possess much importance for the cell-level heterogeneity.
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