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S t is a low-frequency component with a bandwidth of 90 days and with a blending of locally quadratic and locally constant fitting.
Here, we employ a third-order polynomial for the scattering and an approximately constant fitting window.
Boltzmann and time constant fitting procedures are as described previously.
All these methods are referred to as Piecewise Constant Fitting (PCF) algorithms and seek to minimize a penalized least squares criterion.
We use segmentation by the Piecewise Constant Fitting (PCF) algorithm (with a breakpoint penalty parameter of 3) (Baumbusch et al., 2008 ) to determine "switch-points" of haplotype blocks.
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The glycerol permeability coefficient (Pgly) was calculated using the following equation: Pgly = 1/[(S/V)τ], where S is the vesicle surface area, V the cell volume, and τ (K−1) is the exponential time constant fitted to the vesicle swelling phase of the light scattering time course corresponding to glycerol entry39.
In contrast, the regression curves between the shrinkage and initial shrinkage) and overall time constant) fitted well with a second-order polynomial.
4) Time constant fits – it is worrisome that the fitted time constants are in the time range where the current time courses were extrapolated to the origin from later time points.
The fold change in inactivation time constant (fitted with stimulus vs response with variable slope; GraphPad Prism v6) in the presence of vehicle or compound was determined as described (Dubin et al., 2012).
It also shows larger Weiss constants (fitting above 130 K) and lower blocking temperatures, which indicate the surface coating can suppress the deterioration of Li/Ni disorder upon repeated cycles.
Pseudo first-order constants fitted with Monod kinetics were later substituted into the transport model.
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