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Basically the constant fed-batch fermentations were conducted in two phases.
Intermittent and constant fed-batch fermentations were performed in the PFB to optimize the fermentation results.
Constant fed-batch fermentations were conducted in a 2 L stirred tank bioreactor at three different feeding rates of 0.008, 0.015 and 0.03 L/h.
Figure 5 depicted the time course of constant fed-batch fermentation of P. acidilactici in 2 L stirred tank bioreactor with different feeding rate of limiting substrate.
Whilst for the constant fed-batch fermentation, different feeding rates were used to study the effects of feeding rate on viable cells production and lactic acid accumulated from cultivation of P. acidilactici.
Fig. 6 The time course of constant fed-batch fermentation of P. acidilactici in 2 L stirred tank bioreactor with in situ addition of 10 g/L IRA 67 resin.
Fig. 5 The time course of constant fed-batch fermentation of P. acidilactici in 2 L stirred tank bioreactor at feeding rate of a 0.008 L/h, b 0.015 L/h and c 0.03 L/h.
The cultivation performance of P. acidilactici in the constant fed-batch fermentation coupled with extractive fermentation using the condition of dispersed anion-exchange resin were conducted at feeding rate of 0.015 L/h.
Improvement of 6.1 times in maximum viable cell concentration and reduction of 1.6 times in lactic acid production were achieved in constant fed-batch fermentation as compared to batch fermentation.
The maximum viable cell concentration obtained in constant fed-batch fermentation at a feeding rate of 0.015 L/h was 6.1 times higher with 1.6 times reduction in lactic acid accumulation compared to batch fermentation.
The improvement of the P. acidilactici growth in the constant fed-batch fermentation indicated the use of minimal and simple process control equipment is an effective approach for reducing by-product inhibition.
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