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The triggered unit activated a constant current stimulus isolation unit (Neurolog, UK).
The program activated a constant current stimulus isolation unit (Neurolog, UK).
As mentioned above, a Grass Instruments S88 stimulator in series with a Grass Instruments CCU1A constant current stimulus isolation unit was used to deliver electrical stimulations.
We used a Grass Instruments (Quincy, MA) S88 stimulator in series with a Grass Instruments CCU1A constant current stimulus isolation unit to deliver stimulations.
Electrical stimuli were delivered via a theta-glass bipolar electrode filled with ACSF using a constant current stimulus isolator (DS-3, Digitimer, Letchworth Garden City, UK).
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Stimulation used for evoked compound action potentials was constant current stimulus-isolated square wave pulses.
Monophasic, constant-current stimuli (100 µsec) were delivered with a bipolar stainless steel electrode placed in the stratum radiatum of the CA3 region, and the field excitatory postsynaptic potentials (fEPSPs) were recorded in the stratum radiatum of the CA1 region with electrodes filled with ACSF (Re = 2 4 MΩ).
The amplifier then delivered constant-current stimuli through the monopolar recording electrode referenced to ground (Lyons-Warren et al., 2012).
Individual stimulation pulse was a biphasic charge-balanced pulse (0.1 ms each phase) delivered through a constant current stimulator (stimulus isolator A365R, World Precision Instruments, FL), driven by a Master-8-VP stimulator (AMPI, Israel).
A constant monophasic current stimulus (delivered by Isoflex stimulus isolator, AMPI Inc., controlled by Grass Instruments S88 Stimulator) was applied through the bipolar stimulating electrode with the following stimulation parameters: single rectangular pulse for 2 msec, +500 µA amplitude.
Evoked excitatory post-synaptic currents (EPSCs) were recorded in response to 100 μs long constant current pulse stimuli from constant current bipolar stimulus isolator units (A365, World Precision Instruments, Sarasota, Florida, USA).
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