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Electrical stimuli were applied using a constant current device (Neurometer® CPT, Neurotron Inc., Baltimore, MD).
Three constant-current pulses of 0.5 Amps were delivered for 52 msec/pulse separated by a 1 sec delay using the CELLECTRA® adaptive constant current device (VGX Pharmaceuticals, The Woodlands, TX).
The 5-Hz sine wave electrical stimuli were applied via two gold electrodes placed on the medial and lateral side of the mid-phalanx of the left middle finger by means of a constant current device (Neurometer® CPT, Neurotron Inc., Baltimore, MD, USA; maximum output 20 mA).
Current passage through 5 MΩ metal electrodes (stainless steel or tungsten, A-M Systems) was controlled by pClamp software (Axon Instruments) gating a constant current device (AMPI).
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DNA was delivered into the semimebranosous muscle followed by in vivo electroporation using the constant current CELLECTRA® device (VGX Pharmaceuticals, The Woodlands, TX).
ECT was administered using a bidirectional constant current, brief pulse device.
The injection was followed by constant current EP (CELLECTRA® device, VGX Pharmaceuticals Inc., The Woodlands, Texas) at 0.5 Amps, 3 pulses of 52 milliseconds each, 1 second between pulses with an IM applicator consisting of 1.375 ± 0.020 inch long 21-gauge needles in a pentagonal arrangement [ 50].
The on-chip pump can also be readily used as constant flow sources (equivalent to constant current supplies for electronic devices) for routine microfluid manipulations on LOC devices.
The electrical degradation will be referred to as electrical fatigue and is understood as mechanisms, phenomena and material properties that change during continuous operation of the device at constant current.
AC was applied using the device with a constant current output of 30 ± 6 μA and loading resistance of 0 60 kΩ.
Centralized ADC units connected out to field devices supplying a constant current over copper twisted pairs.
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