Based on NOEs, vicinal coupling constants, backbone amide exchange rates, and chemical shift indices, PGAa formed a long amphipathic α-helical conformation in both TFE and DPC micelle environments, spanning the residues Ile2 Ala19 in TFE and Lys5 Ala19 in DPC micelle, respectively.
Antibodies and artificial families of antigen binding proteins (AgBP) are constituted by a connected set of hypervariable (or randomized) residue positions, supported by a constant polypeptide backbone.
An enhanced force constant for backbone-connections (bb-cons) is added to reflect the chemical reality (also used in Hinsen's ENM and BENM, see below) hence the name Chemical Network Model (Kondrashov et al. 2006).
The two cases of hysteretic systems are studied: the system with limited stress when strain increases without bound, and the system with constant stiffness (linear backbone curve) into which hysteretic properties are introduced.
To facilitate analysis of transiently accessible amides, the hydroxide-catalyzed rate constants for every backbone amide of ubiquitin were determined under near physiological conditions.
To investigate if differences in local secondary structure contribute to the HN chemical shift differences observed between Aβ43 and Aβ42, JHN-Hα coupling constants sensitive to backbone ϕ angles were obtained by line-shape analysis of high-resolution H HSQC spectra.
The fluorescence intensity of fluorescein dye attached to the chitosan backbone remains constant as an internal reference, while the Eu3+ emission increased linearly upon the consecutive addition of DPA.
The side chains were then relaxed using several sequential minimizations with the force constants applied to the backbone atoms being decreased in each run.
Under these conditions, it was shown that the composition and the distribution of NIPAM and MADAP units into the macromolecular backbone remain approximately constant until 80% of conversion.
The results showed that the molecular weight of triblock copolymers increased either with the increase of the molar ratio of MMTC in feed while the PEG chain length kept constant, or by lengthening the backbone chain of PEG block with the same ratio of MMTC in feed.
