We present a real-time FRET-based method for probing the reaction cycle of chaperone proteases, which consists of substrate unfolding, translocation into the protease and degradation.
Group 2 (dimethoxylated monophenols) consisted of substrates related to 2,6-DMP, which was examined as the most preferred aromatic substrate of A. chroococcum crude PO preparations.
Constructed wetlands, which are designed ecosystems that consist of substrates, plants and microorganisms, have been used as an alternative clean technology to treat wastewater.
Reactions are primarily contained in metabolic pathway maps and consist of substrates, products and information about reversibility of the reaction.
Inputs to the HSI model consist of substrate distributions from river surveys, and water level and velocity time series, outputs from a hydrodynamic model.
To illustrate applications of the present solution, results are calculated for elastic thermal stresses in thermal barrier coatings, which consist of substrate, graded bond coat, and top coat.
A central composite design with 5 levels and 3 factors consisting of substrate ratio, reaction temperature, and incubation time was used to model and optimize the reaction conditions using response surface methodology.
Cell spreading is a complex process consisting of substrate adherence and passive-descent events, later followed by active probing of the cellular environment [44].
The control consisted of substrate with 0.5% (v/v) ethanol.
Here is a small example of application of above methodology, where we organize the calculation of derivatives for all the isotopomers of system consisting of substrate and product of pdh reaction with constant pyruvate input: v0 → pyr → accoa →.
The reaction was started by the addition of tissue extract and OD340 of samples was measured against a blank consisting of substrate and buffer but with no plant extract.
