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Phosphate buffer solution consists of phosphate, carbonate and chloride while 0.5 mol/L NaCl contains only chloride.
The reaction mixture of 2 mL consists of phosphate buffer (0.1 M, 7.4 pH), 1 mM GSSG, 0.5 mM EDTA, 0.1 mM NADPH, and 10% PMS.
The standard medium used to wash and hold cells in suspension during the isolation procedure consists of phosphate buffered saline (PBS), without Ca2+ and Mg2+, containing 0.3% bovine serum albumin (BSA) (product #100362; Roche Molecular Biochemicals, Indianapolis, IN, http://www.ibuyrmb.com) and 50μg/mL gentamicin sulfate (# 15750, Life Technologies/Gibco BRL, http://www.lifetech.com).
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Our SLF consists of phosphate-buffered saline (PBS) with four antioxidants.
The reaction mixture consisted of phosphate buffer, H2O2 and enzyme extract.
The substrate mixture consisting of phosphate buffer (50 mM) and H2O2 (15 mM) was added to the enzyme extract.
The mobile phase used for separation consisted of phosphate buffer (pH 4): methanol: tetrahydrofuran (70: 10: 20, v/v) pumped at flow rate of 1.0 mL/min.
The eluent system consisted of phosphate buffer (2.4 mM NaH2PO4 + 1.6 mM Na2HPO4; pH 6.8), containing sodium sulphate (25 mM), to achieve an ionic strength of 100 mM.
The trapped fluorescent derivative was back-flush eluted to fluorescence detector with an environmentally friendly mobile phase consisting of phosphate buffer and ethanol.
100 μl of each fraction was mixed with the reagent solution (1.0 ml) consisting of phosphate buffer, 0.6 M H2SO4, 28.0 mM sodium molybdate and 4.0 mM ammonium molybdate.
In the typical experiment, 4 mL of DES buffer reaction system was consisted of phosphate buffer (3.0 mL, 100 mM, pH 6.5), DES (0.4 mL) and enzyme liquid (0.6 mL, 1.92 U).
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