UltrathinCo9S8nanosheets were successfully assembled into a carbon supported nanostructure of C@Co9S8core-shell hollow spheres by a facile solution-based method consisting of template-engaged growth of a precursor shell, followed by simultaneous sulfidation and removal of template.
Whitney's tooling consisted of templates (tool-guiding patterns) and rudimentary fixtures the antecedents of today's tools and dies and he successfully demonstrated the feasibility of manufacturing interchangeable parts by mass-producing firearms for the War of 1812.
Another way to estimate the amplitude of an overlapped harmonic is to use an instrument model, which may consist of templates of spectral envelopes of an instrument [16].
DNA extracted from diet samples is inevitably highly degraded [14], [15] and primarily consists of templates not relevant to diet studies (i.e. predator or gut fauna DNA) [8].
The bottom layer consists of templates (sub-patches) of the images and the intermediate layers consist of secondary templates formed by the assembly of the templates in the lower layers.
The reaction mixture consisted of template cDNA, 0.2 μM of each primer, 300 μM dNTPs, 3 mM MgCl2, and 0.05 U/μl ExTaq DNA polymerase (Takara Shuzo, Ohtsu, Japan).
PCR was performed in a 50 mL reaction mixture that consisted of template DNA, 20 pmol of each primer (Table 3), and Hypernova-RED master mix (DNA Gdańsk) in a Biometra thermocycler (Biometra, Germany).
External oligonucleotides SO1 and SO2 were dephosphorylated and consisted of template-specific sequences and high-melting suppression sequences having no similarity to each other (Table 1, Additional File 1).
For the amplification of GGPPS1 and 18S genes, real-time PCR was done in triplicate for each sample in a 20 μl reaction mixture, which consisted of template DNA (2 μl), primers (900 nmol/L), probe (250 nmol/L), Mg2+ (5 mmol/L) and Ex Taq HS (0.1 units/AL; ExScript Real-time PCR Kit, TaKaRa).
Real-time PCR was performed in triplicate for each sample in a 20-μL-reaction mixture, which consisted of template DNA (2-μL), and primers (900 nM), probe (250 nM), Mg2+ (5 mM), and Ex Taq HS (0.1 U/μL, ExScript™ real-time PCR Kit, TaKaRa).
