Sentence examples for consisting of one cycle from inspiring English sources

The qPCR was run on a Mx3000P QPCR system (Agilent, Santa Clara, CA, USA) with PCR conditions consisting of one cycle of 95°C for 2 minutes, then 40 cycles of 95°C for 30 seconds, 55°C for 30 seconds and 72°C for 30 seconds.

Cycling conditions included 35 cycles of amplification for 30 seconds at 94°C, 30 seconds at 55°C, 1 minute at 72°C, and a final extension phase consisting of one cycle of 10 minutes at 72°C.

The following programme conditions were applied for qrt-PCR running: denaturation programme, consisting of one cycle at 95°C for 8 min; amplification programme, consisting of 45 cycles at 95°C for 5 s, 60°C for 10 s and 72°C for 15 s; melting programme, one cycle at 95°C for 0 s, 40°C for 60 s and 90°C for 0 s; and cooling programme, one cycle at 40°C for 60 s.

All patients received the same high-dose regimen consisting of one cycle of cyclophosphamide 6 g m−2 as a daily 1 h infusion, thiotepa 480 mg m−2 as a twice daily 30 min infusion, and carboplatin 1600 mg m−2 (in case of abnormal kidney function, the carboplatin dosage was based on an area under the curve (AUC) of 20 mg ml−1 min) as a daily 1 h infusion, all divided over 4 consecutive days.

The cycling conditions consisted of one cycle at 95°C for 10 min followed by 40 cycles of 95°C × 15 s, 60°C × 15 s and 72°C × 45 s.

The PCR cycling conditions consisted of one cycle at 95°C for 30 s, followed by 40 cycles at 95°C for 5 s and finally 60°C for 30 s.

The PCR cycling program consisted of one cycle of 95°C for 10 min, 40 cycles of 95°C for 15 s and 60°C for one min, and one cycle of 95°C for 15 s and 60°C for one min (ABI StepOnePlus).

Thermal cycling conditions consisted of one cycle of 30 s at 95°C, followed by 45 cycles of 5 s at 95°C, 10 s at 55 65°C and 30 s at 72°C.

Thermal cycling conditions consisted of one cycle of 1 min at 95°C followed by 40 cycles of 5 sec at 95°C, 10 sec at 61°C, and 20 sec at 72°C.

Thermal cycling conditions consisted of one cycle of 30 sec at 95°C followed by 45 cycles of 5 sec at 95°C, 10 sec at 55 61°C, and 20 sec at 72°C.

The thermal cycling conditions consisted of one cycle each for 2 min at 50°C and 10 min at 95°C and 40 cycles for 15 sec at 95°C and 1 min at 60°C.