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Although we ran a standard curve consisting of dilutions from 100% down to 0.1% for the C allele, we were only reliably able to detect 5% or greater heteroplasmy using SNaPshot.
All samples were counted together, followed by standards consisting of dilutions of the patient injectate.
Quantification of adenovirus copy number was performed using a standard curve consisting of dilutions of adenovirus DNA from 1,500,000 to 15 copies in 10 ng of cellular genomic DNA.
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Relative copy numbers were titrated against external standards consisting of dilution series of plasmids (10 – 10 copies) harboring the respective amplicons.
The inoculum consisted of dilutions of the original pig feces from which the virus was recovered.
The standard curve consisted of dilutions of recombinant human OPG (Alexis Corp).
The procedure consisted of dilution of an aliquot of the extract (up to 100 μL) with water to 600 μL and addition of 60 μL of Folin-Ciocalteau reagent.
In this modeling approach, the input variables consist of dilution ratio "R v," temperature "T," and molecular weight of solvent "M w," while the amount of asphaltene precipitation "W t " was regarded as the target value.
Purified PCR products were used to generate the relative standard curves, consisting of serial dilutions.
A standard curves consisting of serial dilutions from 101 to 106 copy of plasmids containing each cDNAs were included in every assays.
For each experimental setup, a standard curve was prepared consisting of 5 dilutions of PCR products.
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