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The reaction was completed with a melt curve analysis consisting of a melt gradient from 65°C to 95°C, increasing in 1°C increments every 5 s, to discriminate the alleles and to confirm the specificity of amplification and lack of primer-dimer in the real-time PCR reaction.
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Micrometric trilayer ribbons have been prepared consisting of a melt-spun Co-base ultrasoft amorphous nucleus surrounded by two electroplated magnetically harder polycrystalline CoNi layers.
The third segment consisted of a melting curve program.
The reaction cycle consisted of a melting step of 50°C for 2 min then 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. Specificity of the PCR reactions was assessed by a melting curve analysis for each PCR reaction using Dissociation Curves software [ 54].
The bioavailability of propranolol from a matrix-in-cylinder system for sustained drug delivery, consisting of a hot-melt extruded ethylcellulose pipe surrounding a drug-containing HPMC-Gelucire® 44/14 core, was determined.
Thereafter, 45 cycles consisting of a 5 s melt at 95°C, followed by a 30 s annealing/extension at 60°C were performed.
PCR reactions were performed using a total of 50 cycles consisting of a 15 s melt at 95°C, followed by a 1 min annealing/extension at 60°C for CK19, CEA, VEGF-C, hnRNP A2/B1 and β-actin or a 1 min annealing/extension at 56°C for LunX.
In a melt consisting of a single component, such as CaSiTiO5, all atoms could add to titanite nuclei to form crystals of titanite.
Coaxial core-sheath flows consisting of a cylindrical jet of melting RT27 (core fluid) and annular jet of poly vinyl butyral) (PVB) solution (sheath fluid) are formed in a coaxial microdevice.
At compositions beyond 60 mol% of the flexible ethylene glycol unit, a biphasic melt consisting of a predominant isotropic phase was observed.
The PCR reaction was finished by melting curve analysis consisting of a denaturation at 95°C for 15 seconds, melting start at 60°C for 15 seconds followed by a continual increase of temperature up to 95°C for 20 minutes and final denaturation at 95°C for 15 seconds.
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