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L318F was not added because it is not consistently sequenced during surveillance studies.
Some coverage effects were seen, with sequences near the origin of replication consistently sequenced to a higher depth [ 53], than others but the genome of each assembly was adequately covered.
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The analysis of the distribution of reads showed that some genomic bins were consistently not sequenced in all samples sharing the same sampling area, whereas reads corresponding to the same bin were present in samples coming from other regions.
Consistently, when we sequenced the srtF cluster in these strains, the same ochre nonsense mutation found in strain P1/7 was detected in the sfp2 gene (GenBank Accession numbers GQ279101 to GQ279107).
All other FIPVs and all sequenced FECVs consistently had a serine at this position.
Consistently with sRNA sequencing data, qRT-PCR results showed that 6 miRNAs, miR159, miR169, miR319, miR394, miR1919, and miRn1, were upregulated at each examined time point after B. cinerea inoculation.
Peter James showed that a diversion this year into ghost stories with The House on Cold Hill had not diverted energy from his consistently impressive sequence of DS Roy Grace policiers, the 11th of which, You Are Dead (Macmillan), confidently combines a cold case with a very hot one.
In spite of initially using primers designed for Gammaproteobacteria, we consistently amplified sequences from a different bacterial phylum: Bacteroidetes.
Although catalytic activity correlates less consistently with sequence conservation in these extensively randomized proteins, less extreme mutagenesis strategies might be adopted in practice to augment stability while maintaining function.
Only contigs that consistently yielded sequences with higher than 40 phred quality score at all sites (according to the MACE alignment) were subjected to SNP calling.
Second, the set of all clusters must be orderable consistently with sequence orders of their residues.
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