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Further, carboxylate-terminated nanocarriers consistently bound to unoxidized LDL (Relative Electrophoretic Mobility, REM=1.0) and mildly oxidized LDL (REM=1.5), but not highly oxidized LDL (REM=3.6), whereas the neutral nanocarriers displayed no preference/affinity at all, indicating that the nanocarrier-LDL binding is charge-dependent.
This produced a final set of consistently bound high confidence regions for each of the cell lines used in the study.
In contrast, collagelin consistently bound to laminin, previously identified as a GPVI accessory ligand [16]; the sites of GPVI that interact with laminin and collagen thus share a common structure that is mimicked by collagelin.
HCT116 cells consistently bound, as well as processed into larger-MW bands, radioactivity from all three 32P-labeled peptides (MA11, MA20, and MA22) at a much lower rate than Caco2 and HCT15 cells, but incorporation into HCT116 cellular proteins was eventually visible on longer exposures (data not shown).
Improvements in HRQoL were not consistently bound to weight reduction.
The identification of Chk1 as a Wip1 target followed co-immunoprecipitation experiments in our laboratory that showed that Wip1 consistently bound Chk1 in cells [ 10].
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Significantly, it was found that only when challenged with normal oxygen at room temperature did 11168-O consistently bind to sialic acid or terminal mannose structures, while 11168-GS bound these structures regardless of growth/maintenance conditions.
Of the five dynamin GTPases examined, only Dnm1 and Mfn1 consistently bind to GTP at detectable levels in this assay when expressed alone (Fig. 6).
A recombinant fragment of SF1 consistently binds to UAC UA AC and the mutated sequences in a similar manner as long as the two critical residues are conserved (Berglund et al. 1997).
As none of the antibodies against CD98hc could consistently bind to ΔCD98hc-β geo fusion protein, we used the anti-β galactosidase monoclonal antibody for the flow cytometry analysis to examine the cell surface expression of ΔCD98hc-β geo fusion protein.
Further, analysis of glycan array data of human-adapted influenza viruses revealed them to consistently bind to long α2-6 sialylated glycans (tetrasaccharide or longer), despite showing a remarkable overall heterogeneity in their glycan binding patterns, [ 28, 30- 36] (summarized in Additional file 2).
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