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The loss of catalytic activity when these residues were mutated is consistent with this assignment (Table 2).
MCD and EPR spectroscopies are consistent with this assignment and support ligation by an anionic (tyrosinate) group.
Consistent with this assignment, we did not find O-phosphotyrosine in the hemolymph metabolome of adult flies (data not shown).
The proton on the carboxylate group was located on the difference map and the bond lengths within the carboxylic acid group are consistent with this assignment.
On the other hand, sequential Edman degradation of the protein in the band assigned as I-EC revealed that the first 10 residues do indeed correspond to the sequence of the intein consistent with this assignment.
In addition, the expression patterns, evolutionary profile and chromosomal distribution of the genes encoding these peptides are consistent with this assignment in allowing evolutionary flexibility to respond to biotic challenges.
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The finding that the rate of appearance of the 420 nm band measured at pH 6.0 shows that a hyperbolic dependence on the concentration of L-Dopa is consistent with the assignment of this absorbance band to a 4′-N-protonated external aldimine.
This is consistent with the assignment for the intensities of these peaks serving as markers for distorted coordination geometries for terminal and/or defect atom sites, which in turn make proportionally smaller contributions with the increase in domain size following the sequence CoPi, CoMPi to CoBi.
However, the second segment composed of 11 residues in this region is consistent with the assignment in the wwPDB file.
This difference is thus indicated to result from an opposite configuration at the C-15 meso position, between the (substituted) pyrrole rings C and D. This conclusion is consistent with the assignment of the broad absorption bands at longest wavelengths (i.e. at 274 nm and 238 nm) to these two rings[ 18] in the spectra of 1 and of the Hv-UCCs.
The great majority of clustered viral sequences were most similar to known bacteriophage, with smaller representation from eukaryotic viruses (89% total) (Fig. 1A) (Tables S2, S3, S4, S5), indicating that the majority of sequences identified here as viral were clustered in a fashion consistent with this taxonomic assignment.
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