The theoretical predictions based on this effect are consistent with the quantum yields measured experimentally.
This is consistent with the fermentation yields not being as large as 2-fold difference between the transgenic and control lines.
However, in both cases 20 30 % loss of material (consistent with the reported yields 14b of 60 70 %) without any increase in purity (H NMR; see the Supporting Information) or change in the performance of the reagent was seen.
The hydrolysis conditions were optimised by using a central composite design (CCD) as follows: reaction time 4.02 h, temperature 84.35 °C, and H2O2 concentration 2.46%, under which the yield of Chinese yam derived oligosaccharides (CYOs) reached 11.73%, which was consistent with the predicted yield by analysis of the results of CCD (11.89%).
The predicted extraction yield of zerumbone was 5.946 mg/g DM, which was consistent with the experimental yield of 5.88 mg/g DM.
The optimum extraction conditions are as follows: extraction temperature, 100 °C; ratio of water to raw material, 26.56; extraction time, 3 h; and extraction frequency, 2. Under these conditions, the experimental yield of polysaccharides was 13.0 ± 0.25%, which was consistent with the predicted yield.
Under these conditions, the maximal yield of crude HDPs was 25.12 ± 0.145 mg/g DW, which is consistent with the predictive yield of 25.33 mg/g DW.
Under optimized conditions, the experimental yield of P. nigricans mycelia polysaccharides was 15.33 ± 0.21%, which is consistent with the predicted yield.
The yield of the four iridoid glycosides under the optimal process was found to be 63.08 mg/g, which was consistent with the predicted yield.
This result is consistent with the county yield variation (Fig. 3).
