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Several of the newly-added sequences arise in the tree as polytomies, a result consistent with the difficulties inherent in constructing a multigene phylogeny across a broad sampling of eukaryotes.
This interpretation of the damage to epidermal cells is consistent with the difficulties experienced in the ecdysis, pupation, and eclosion that led to death (see above).
Consistent with the difficulties reported in accessing conventional health services, accessing CAM practitioners can also be problematic for older women who may not be able to drive, either because they previously relied on their husband, or because their licence to drive has been revoked.
The fragment specific estimates of template numbers are consistent with the estimates based on limiting dilution (see Figure 9, rank correlation around p = 0.5 for most of the fragments), but indicated that variant recovery in fragment F5 was sometimes poor, consistent with the difficulties we encountered when amplifying fragment F5.
Two-source capture-recapture estimates for the true numbers of cases in the three cities were 71, 82 and 89, implying relatively low completeness estimates of 77%, 54% and 31%, consistent with the difficulties of cancer registration in a huge and developing country.
Length and sequence conservation outside of the SPFH domain varied considerably (see Fig. 1a and Additional file 3) between the different family members and is consistent with the difficulties encountered when trying to compile a phylogenetic tree as mentioned by Rivera-Milla et al. [ 4].
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The tight binding of putrescine to human AdoMetDC is also consistent with the difficulty in removing putrescine from the enzyme.
The relatively low TaEPSPS-4A1 mRNA levels were consistent with the difficulty recovering the TaEPSPS-4A1 cDNA using the F3-R1 pairer pair that was able to bind all three transcripts.
These properties are consistent with the difficulty of activating CRISPR spacer uptake in the laboratory, but appear to conflict with the high complexity and diversity of the CRISPR immune systems that are found among the Sulfolobales.
Finally, only a few studies have provided direct evidence of urban transmission (vector feeding and vector infection), consistent with the difficulty of obtaining evidence for transmission in urban settings.
Sixty percent of respondents who had not had their eyes tested in the past two years said the main reason for not going was that they had not had a problem with their eyes, which is consistent with the difficulty with judgements about changing eyesight noted in this study.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com