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The assay results indicated that deletion of the PAK-J type III secreted exotoxins reduces cytotoxicity by approximately 70%, which is consistent with the assay result of lactate dehydrogenase release assay (data not shown).
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The electrochemical results in NaCl were consistent with the assays of SCC resistance of alloys B and C previously reported in the literature.
Consistent with the assays using P-labeled ATP, uptake of S-labeled PAPS into empty vesicles could not be detected at all (Fig. 3B).
For all screen hits, waking activity was calculated as the number of beam crossings/min when the fly was awake; consistent with the assays performed in the Flybowl, none of the lines had discernable locomotor defects.
Whilst the overall trends in the relative chaperone ability of the wild-type and mutant proteins were consistent with this assay, the absolute percentage protection afforded by the chaperones was found to vary between assays.
Again, this is consistent with interpreting the assay as only measuring activity from Sod1.
qRT-PCR assay showed that the level of Caspase9 was decreased in co-transfection group, which is consistent with the TUNEL assay.
Consistent with the fractionation assay results, BRD9 binding was markedly diminished upon inhibitor treatment (Fig. 6c).
The result is consistent with the cytotoxicity assay (Fig. 6).
Consistent with the PPL assay, the antithrombin concentration significantly decreased in septic rats compared to sham rats (Fig. 1b).
Consistent with the cytotoxicity assay, FPCC induced higher fraction of early as well as late apoptosis cells.
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