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These findings are consistent with sumoylation of FOXL2 in vivo.
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Consistent with SUMOylation being downstream of phosphorylation we found that activating PKC with phorbol ester enhanced GluK2 SUMOylation.
Consistent with SLX4 SUMOylation, the mSLX4 nuclear bodies were enriched for SUMO-2/3 (97.9% co-localization) (Fig 3B).
The consistent SUMOylation of the centromeric heterochromatin of human chromosome 9 is noteworthy because of this unusual genomic structure.
We are able to detect transcriptional coactivator effects of ectopic lipin-1α in neuronal cells, and show that sumoylation is required for this activity, consistent with the effects of sumoylation on the nuclear localization of lipin-1α.
Consistent with a role of sumoylation in inhibiting Tec1 activity, specifically increasing sumoylation of Tec1 by fusing it to the sumoylating enzyme Ubc9 leads to a dramatic decrease of Tec1 transcriptional activity.
This result is consistent with the absence of the major sites of SUMOylation in v-Myb due to C-terminal truncation of the protein (Fig. 1).
This result is consistent with other reports that SUMOylation of p300 and CBP may suppress coactivator activities, possibly through recruitment of HDAC complexes [12], and that a SUMO-defective CBP showed increased coactivator activity in IRF1 reporter assays [10].
Other sumoylation targets identified in our screen, including PP2A, Arp3, Cofilin (Twinstar), Mago Nashi, and Profilin, are also consistent with multiple roles of SUMO in mitosis.
This possibility was consistent with the follow-up studies of sumoylation of recombinant human topo I that showed a reduction of catalytic function.
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