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The distribution of functional groups (functionality maps) on the substrate binding site allows for identification of modifications of the tetrapeptide sequence that are consistent with potent peptidic inhibitors.
After 3 4 minutes of pulsation, minimum surface tensions of Infasurf preparations reached approximately 3 mN/m, consistent with potent surfactant activity.
Compound 1 inhibited TNFα-induced activation of NFκB-Luc in HEK-293 cells with an IC50 of 47±7.7 nM (mean±S.E.M., n=7), consistent with potent proteasome inhibition in these cells.
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Consistent with its potent antiproliferative activity, 4f also displayed excellent antitubulin activity (IC50 = 0.77 μM).
Consistent with a potent response to NAD+ [37], recruitment of MLX, MondoA and Pol II to the Txnip promoter was increased under NAD+ treatment in U2OS cells (Figure S7E).
Key results included the identification of a limited number of induced genes mainly involved in cell development, cell proliferation and signal transduction, consistent with the potent effects of these fusions on promoting primitive hematopoietic cell expansion and differentiation block in vitro and their ability to collaborate in leukemic transformation [19], [21], [27].
γIR treatment promoted significant nuclear accumulation of p53 consistent with a potent p21-dependent cell cycle arrest phenotype.
LPS also induced an increase in plasma FFA concentrations within 1 h, which continued for 24 h, consistent with a potent lipolytic effect of LPS.
Many of these quantitative differences in gene expression efficacy are consistent with the potent agonist activity of EE and the weak agonist activity of TAM.
Consistent with the potent effects of these factors in the mouse system, we found that combined overexpression of KLF2 and NANOG in primed human ESCs resulted in increased OCT4-ΔPE-GFP reporter levels in a fraction of cells.
Real-time qPCR showed induction of HO in all infected fish, consistent with its potent cytoprotective and anti-inflammatory functions [ 41], but the expression level was greater in LR.
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