The qRT-PCR results were also consistent with the normalized microarray data.
As shown in Fig. 6, the fold-changes in gene expression determined by QPCR were quite consistent with their normalized read counts (expression level) determined by RNA-seq.
Of the different miRNAs studied, miR169 had the least expression in both tissues and only a faint signal was detected, which is again consistent with the normalized read count.
Biochemical model outputs were also selected to be consistent with the normalized-Hill model species as closely as possible.
When normalized to body weight, oxygen consumption of SirT1-null mice was higher than that of controls, indicating that they are hypermetabolic (Figure 4A, B), a conclusion consistent with their normal food ingestion and lower body mass.
These results are consistent with the non-normalized set of data for these same experiments as both show the same trends in luciferase activities (compare Fig. 4 to supplementary material Fig. S4); this is an important observation as interference with the expression of the control reporter plasmid can result in biased interpretation of the normalized data (Vesuna et al., 2005).
Efficiencies of conversion of the anionic first block to diblock were as high as 33%100%0% normalized), consistent with the known 31 Li-R/Ti relationship in the active catalyst species.
Hence, in patients with an increase in skeletal muscle signal intensity ≥ 20%%, as well as in patients with a recent history of muscular pain, an increase of > 45%% in absolute myocardial signal intensity between pre- and post-gadolinium images is suggested as a threshold consistent with myocarditis, rather than normalized EGEr [11].
Overall, the LH response to kisspeptin was more limited than that achieved in healthy men using the same protocol (George et al., 2011) with lower LH mass per pulse, although pulse frequency was normalized, consistent with complex neuropeptide interactions associated with KNDy neurone function rather than a linear hierarchy, as described above.
