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All of the above data are consistent with depletion of Rad21 resulting in the arrest of only a minor fraction of cells in mitosis with separated sister chromatids.
Furthermore, the observed down-regulation of pfk-pyk (EF1045-46) and fba (EF1167) involved in the first steps of the glycolysis is also consistent with depletion of intracellular glucose catabolic intermediates.
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There is also a study showed that the anti-CD25 mAb selectively depleted cells with highest level of CD25 expression, and the changed intensity of immune response was more consistent with the depletion of Treg cells than that of effector T cells [37].
When we acutely perturbed Arp2/3 activity using the selective inhibitor CK666 [ 20], we observed a phenotype with small blebs in ∼60% of cells, consistent with depletion experiments, thus suggesting that CK666 is a good substitute for gene depletion.
This hypothesis is consistent with the depletion of the ER at late stages of autosis and the presence of membrane-bound structures in the perinuclear space.
The accumulation of PEP in the XI-strain suggests a reduced activity of PYK which is consistent with the depletion of FBP.
Consistent with the depletion of ergosterol in the plasma membrane of Δ pdr18 cells, PDR18 expression was also found to be essential in the maintenance of yeast plasma membrane potential.
The abundance of Group I particles is consistent with the depletion of 3′-domain proteins (S2, S3, S7, S9, S10, S13, S14, S19) observed by qMS.
The injection of anti-CD25 Abs neutralized most of the CD4+CD25+Foxp3+ fraction and these were consistent with the depletion of CD4+CD25+ T cells (Fig. 4).
The greater reduction of various autoantibodies with dual immunotherapy is consistent with greater depletion of various B cell subsets.
Consistent with this, depletion of Treg cells using PC61 during the first 2 days of pregnancy led to implantation failure (Zenclussen et al., 2005).
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