The observed kinetics is consistent with bi-layer formation observed with, for example, the quartz crystal microbalance with dissipation (QCM-D) and the surface plasmon resonance (SPR) techniques.
Microtubule-dependent kinetochore stretching after depletion of condensin would be consistent with bi-oriented attachment and subsequent stretching of a more "elastic" centromere/kinetochore in the absence of condensin.
Off-line HPLC MS analysis afforded m/z 911 [M H]− for peak '1', consistent with bis-glutathionyl S-quercetin, while peaks '2' and '3' furnished m/z 606 [M H]−, suggestive of glutathionyl- S-quercetin.
Epigenetic inactivation by methylation and a reduction in expression would be consistent with bi-allelic inactivation according to Knudson's two-hit hypothesis.
The full complement of high-Man N-glycans are present (m/ z 1579.8, 1783.9, 1988.0, 2192.1 and 2396.2) and a number of complex glycans can be identified, with antennal arrangements consistent with bi-, tri- and tetra-antennary forms.
Although previous resonance Raman study on the ferric VHA-Mb were not conclusive for its coordination structure, present EPR parameters of the ferric VHA-Mb were consistent with bis-imidazole coordination of His68/His97.
Although the helicity of the free peptide was low, upon ligatation of the heme, the helicity was seen by CD to increase to ∼50%, and the electronic spectra were consistent with bis-His heme ligation, similar to b-type cytochromes.
A non-local estimate of the mean value of the angle of twist of the beam leads to a shear gradient that is energetically consistent with a bi-moment, in the spirit of the averaging theory of Vardoulakis and Giannakopoulos (2006).
This finding is consistent with reported bi-phasic actions of cyPGs, whereby they increase proliferation of cultured cells at ∼1 µM [37], [38], while they decrease proliferation or cause apoptosis by modifying other protein targets at ∼50 µM [39].
This polarized distribution is consistent with SR-BI being a receptor for basolateral to apical cholesterol transport into the bile.
