Sentence examples for consistent transcript levels from inspiring English sources
Actin was selected for normalization due to its consistent transcript levels throughout leaf and fruit tissues, with crossing threshold (Ct) values changing by less than 2. For each gene, a standard curve was generated using a cDNA serial dilution, and the resultant PCR efficiency calculations (ranging between 1.839 and 1.945) were used for relative transcript level analysis.
This was selected as a reference gene because of its consistent transcript level among varieties.
Actin was selected for normalization due to its consistent transcript level throughout fruit tissues, with crossing threshold (Ct) values changing by < 2.
The expression was normalized to Fragaria × ananassa actin and Prunus cerasus actin with Fragaria vesca stage 1 flower bud and Prunus avium 'Rainier' stage 1 fruitlet acting as calibrator with a nominal value of 1. Actin was selected as a reference gene because of its consistent transcript level throughout fruits and leaves.
Consistent with transcript levels, DNA methylation in liver tissue was not significantly changed comparing WT and Lsh−/− samples.
Seed storage protein levels were consistent with transcript levels.
In addition, protein expression of integrin β3 (ITGB3) was consistent with transcript levels determined by microarray.
Membrane expression of IGF-1R, as determined by flow cytometry, was consistent with transcript levels, whereas expression of IGF-2R and IR did not vary among cell lines (Supplementary Figure S2, panel b); however, it should be emphasised that IR-A and IR-B levels could not be determined separately, due to the lack of antibodies able to discriminate between the two isoforms.
Two genes with available antibodies, Aquaporin 1 and Topoisomerase 2A, showed protein expression consistent with transcript level predictions.
Consistent with the transcript levels, we found that the levels of the RelB protein were significantly decreased in resistant male CLL cells when compared with sensitive cells.
Consistent with blood transcript levels, the relative expressions of MT-1A and MT-2A were significantly lower in arsenicosis patients than in healthy subjects.
