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Thus, sample preparation must be optimized to ensure accurate and consistent protein quantitation of the starting samples.
Profiling of the proteins expressed in spikelets using a 2D-PAGE strategy revealed the presence of approximately 500 clear and consistent protein spots, of which 29 spots showed a differential expression to water deficit, heat, and/or combined stress.
Compared to scRNA-seq alone, CITE-Seq demonstrates both the highly consistent protein and RNA profiles with literature and also an enhancement of characterization of cell phenotypes based on immune cell experiments.
The spectra were internally calibrated with consistent protein peaks of m/z values of 8330 and 4165.
The results give proof of principle for consistent protein expression upon RMCE.
4. Stain membranes with Ponceau S to confirm consistent protein loading (Romero-Calvo et al., 2010 ).
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Co-immunoprecipitation results demonstrate a direct interaction between these proteins with a time course consistent with protein redistribution as shown by immunofluorescence data.
Indeed, we detected some proteins in the certain tissues whose transcript levels were not consistent with protein expression levels.
Consistent with protein accumulation, the HT blot revealed an increase in the amount of expressed HT tagged protein for all variants in the presence of MG132.
The PCR product profiles indicated the presence of genes encoding Bin A, Bin B and Mtx1 in all analyzed strains; they are consistent with protein profiles.
The influx rate constant of 11C-metformin in renal cortex (K 1) was 2.2-fold lower in transgenic mice whereas the backflux rate constant (k 2) was similar in the two groups, consistent with protein expression.
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