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These consistent probe sets, which passed this filter, were further used to calculate gene expression changes between different conditions.
AffyProbeMiner has provided transcript consistent and gene consistent probe sets by clustering probes matching the same set of transcripts and genes [ 4].
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We then obtained the intersection of the population-consistent probe sets across YRIp and CEUp identifying 1043 such probe sets.
This resulted in the following sets of population-consistent probe sets: YRIp 3121 probe sets, CEUp 2759 probe sets, YRIc 1640 probe sets and CEUc with 1520 probe sets whose combined expression ranges were within a one standard deviation band spanning the population mean.
The decreases seen when comparing the 0.1 μg to the 0.5 μg samples were not associated with distance from the 3' end and were not consistent for particular probe sets (only 26 probe sets decreased in at least 7 of the 8 samples).
We conclude based on these results that the genome-based grouping and screening method yields more consistent results for redundant probe sets.
Consistent with this idea, probe sets with the same signal level alterations, regardless of the sample handling protocol applied, are of particular interest.
A cluster "averaging" over probe sets consistent with the SCOREM algorithm, recently proposed to handle such redundant probe sets [ 27], was used.
A likely cause for the consistent decreases of particular probe sets in the amplified samples, not related to low signal level, is the loss of the 5' end of the transcript.
To study only genes that showed consistent expression on experimental chips, probe sets that did not show consistent present calls in the experimental duplicates were removed.
The simulated distribution of average ranks from probe sets with consistent ranks within probe sets was obtained by assigning succeeding ranks to probes from the same probe sets.
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