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Given the consistent insertion pattern, it is tempting to speculate that there may be rules governing the types of mutations that are possible for a given target.
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This pattern of gene expression is consistent with insertion of the L4 cassette at the LoxP site as planned.
Consistent with insertion into active chromatin we have determined that SVAs are more prevalent in genic regions compared to gene deserts.
In each case, the size of the amplified product was consistent with insertion of the provirus into the hnRNPA2/B1 gene (data not shown).
Only the first 15 bp of the hsp70S3 71 5'-UTR could be aligned; the UTR is disrupted by large blocks of repetitive sequences upstream of this position, consistent with insertion and/or rearrangement via transposition.
In addition, despite the fact that retrogenes use machinery of transposable elements, they do not follow the transposable elements genome location pattern recently described in detail [ 20]: high density in the X chromosome and consistent bias insertion close to female and male germline expressed genes.
In two cases (highlighted by a white arrow) a single PCR product is seen which is consistent with intron insertion in all three potential insertion sites.
Thus, all Alu insertions, including the truncated ones, in the data set are consistent with proper insertion events generated through the enzymatic machinery of L1.
This de novo piRNA production produces a characteristic signature on the left and right flanking regions of novel TE insertions, consistent with bidirectional transcription from the novel TE insertion.
A sound absorbent median noise barrier 1m in height produced consistent values of insertion loss of between 1 and 2dB over the range of receiver positions and ground conditions considered.
When the median barrier was used in conjunction with a roadside barrier it produced a consistent improvement in insertion loss of between 1 and 2 dB over the range of conditions considered.
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