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We used a discriminant analysis method of image thresholding based on the density histogram of a selected ROI to ensure consistent image thresholding across all subjects studied.
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Sezgin, H. & Sankur, B. Survey over image thresholding techniques and quantitative performance evaluation.
However, the image segmentation results depend heavily on the chosen image thresholding methods.
All the same, a consistent image emerges.
Image thresholding is an important field in image processing.
Supplementary Figure 2 Procedure for image thresholding and deconvolution.
Try to maintain a consistent image of yourself.
Blood vessels are identified in the image by thresholding techniques.
Each reference image was thresholded by the background value obtained from the images of uninfected embryos.
Data were consistent from image to image.
A resulting statistical image was thresholded at Z>2.3.Two hundred VSTs were identified.
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