For temperature consistent culture, cells were inoculated into freshly prepared medium and cultured at the indicated temperatures to a stationary phase for collection.
"When you see that type of turnover over and over and over, it's impossible to create any kind of sustainable consistent culture".
An independent report released in early May, one of many commissioned by the BBC in the aftermath of the Savile scandal, painted a picture of an organization with a "visible, frequent and consistent" culture of bullying, anxiety and fear.
Critics as well as some members of the bank's current management say the firm never merged these disparate assets into one consistent culture, and that the headlong pursuit of growth at any cost trumped ethical and legal behavior, especially in the investment bank.
She cited "a consistent culture of sexism and sexual harassment," and accused them of wrongful termination and infliction of emotional stress, among other things.
Sub-cultures were taken every 2 3 d to establish consistent cultures for ongoing experiments.
The IV population has been maintained under consistent culture conditions for over 700 generations, and we emulated the culture protocol in almost every detail for our fitness assays.
Since the supply of available nutrients and growth factors may vary in post-confluent cultures, the use of serum-depleted media may offer a more consistent culture environment to evaluate the role of nutrients and serum growth factors in CD26 expression.
Further, it is vital to understand the acceptability amongst primary care professionals regarding potential increased workloads from a new approach which may drive patients towards primary health care [ 22], particularly in a context where there is no existing screening programme or consistent culture of screening for chlamydia.
The rate of entry of rad52∆ est1∆ cultures into senescence was much slower than that observed in liquid cultures, consistent with cultures being diluted less on solid agar compared with liquid cultures and, therefore, having less potential to divide.
