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The changes seen are highly consistent between cases, and are independent of tumour type (carcinoma versus adenoma) or the presence of a germline TP53 mutation.
In the cerebellar populations and motor neurons but not hippocampal neurons, there was a difference between the frequency of antisense and sense foci which was consistent between cases (representative images are shown in Fig. 1a).
Under-recording should not affect LRs (which underpin PPVs) as long as it is consistent between cases and controls; we have no reason to think this is not the case.
Under-recording should not affect likelihood ratios (which underpin PPVs) as long as it is consistent between cases and controls, but may lead to an overestimation of PPVs, and they will only remain valid if GP recording and patient consultation behaviour do not change with time (Hamilton, 2012; Shapley et al, 2010).
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Another important consideration is that the current study focused on plaque pathology in the frontal cortex and had we sampled regions in other vulnerable areas, particularly the entorhinal cortex (which was not available for all cases), we may have observed OC levels that are more consistent between MCI/CIND cases and AD cases.
This test served as a first cut to eliminate assays that did not show a consistent difference between cases and controls with minimal use of case and control specimens.
We evaluated a 45-probe-set containing unique genes related with the principal altered cell signaling pathways in leukemia according to data bases (GeneCards, NCBI, etc).. Normalized ΔCq values for 19 genes showed consistent differences between cases and controls at least until the second month of follow-up.
Subsequently, 11 trained psychiatrists independently rated these three cases; the numbers of depressive symptoms listed in the DSM-IV-TR in the melancholic, reactive, and one-in-between cases were fairly consistent among the raters with 5.1 ± 0.3, 5.1 ± 0.3, and 4.9 ± 0.3 (mean ± SD), respectively (detailed data available upon request).
To maintain consistent exclusion criteria between cases and the sub-cohort, subjects who died during the neonatal period who were born in 1980 (the earliest year neonatal deaths were recorded) or later were excluded.
The tumour-specific methylation changes at the HES5 promoter were consistent within and between cases and comparable with the hypermethylation observed at the GSTP1 gene (Fig. 1d and e), which is invariably silenced in prostate cancer and has been extensively studied (Lee et al. 1994).
Eigenshapes and oscillations are remarkably consistent between these different cases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com