Exact(1)
Phylogenetic analyses of individual protein sequences (supplementary fig. S3, Supplementary Material online, and see Neville et al. 2012) represented as a galled network in figure 5 reveal the consistent affinity of listerial flagellar-system proteins for their homologs from the B. cereus complex, where the corresponding genes are similarly clustered in a tight genomic interval.
Similar(59)
We confirm the existence of consistent affinities between assemblages that were thought to belong to different supergroups of eukaryotes, thus not sharing a close evolutionary history.
Aggregation of species or genus pan-genomes would be another option, removing most members of homologous sets that have consistent affinities with other lineages (e.g., ribosomal proteins).
CR6261 neutralized all H1 clade and H9 clade viruses, except for one of the H2N2 isolates, and potency among subtypes was relatively well conserved (Fig. 5c) consistent with affinity measurements (Table 3).
Given the fact that the in vitro recovery of 3-MT across the dialysis membrane at present conditions is about 20% (data not shown), we estimate that behaviorally active extracellular 3-MT concentrations should be above 500 nM which is consistent with affinity values of 3-MT at TAAR1 in cellular assays ([16], [20], [21], [22], Figure 2).
With Mixtures B and C, the consistent relative affinity for each of the four candidate biotin derivatives was also obtained (Table 3).
The equilibrium dissociation constant thus derived was 1 mM, a calculated KD within the range reported for peptides mimicking the HSP72-interactive domain of p53 (i.e., <200 mM) and consistent with affinity values reported for well-characterized HSP72-mediated activity control reactions (6, 20, 29).
For each candidate biotin derivative, different methods yielded consistent relative affinities.
Modifications of the linker length resulted in weakened inhibitory affinity, consistent with the geometric requirements of an enzyme-catalyzed dissociative transition state.
These in vitro results are consistent with binding affinity as the Bi-NonGly fusion toxin binds strongest to CD25 on the same LCL13271 cells.
This means that it takes 6.79 residence times to flush out Na, 9.25/9.38 residence time for Ca/Mg, and 20.09/18.76 for Ba/Sr, which are consistent with their affinity to the solid surface.
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