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All reverse primers were PIG-tailed by adding GTTTCTT to obtain consistent addition of adenine by Taq DNA polymerase (Brownstein et al., 1996).
We tested this null hypothesis of consistent addition of new states using a Mann-Whitney test (Mann & Whitney, 1947) to compare the ranks of the number of steps in the two categories.
Finally, the non-linear remainder of the data was used to test the null hypothesis of consistent addition of states, by categorizing steps into two categories: those that added new states and those that did not.
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Initial velocity and inhibition studies of the enzyme from rat and P. chrysogenum were consistent with an ordered addition of substrates in each reaction direction [ 36, 43].
For SOXL 1 treated lanes, an additional band consistent with addition of at least one copy of SOXL 1 (∼1.6 kDa) to GST monomer (∼27 kDa) is apparent.
It is assumed that an instantaneous b1 concentration jump initiates formation of B*, consistent with addition of an exogenous aBH3 or dBH3 domain peptide to isolated mitochondria.
Therefore, both studies are consistent - addition of night-time intensivists to a high-intensity ICU does not make a difference in mortality or length of stay.
Importantly, the migration rate of this protein species was slower compared to that of unmodified Tpz1, consistent with addition of SUMO to Tpz1.
The analysis identified a peptide with a mass consistent with addition of Photo-probe [ m/ z 1574.8 (inset c in Figure 4A)].
Ubiquitin increases the apparent mass of Perforin-2-GFP by ∼10 kDa which, given some uncertainty in mass estimation, is consistent the addition of a ubiquitin monomer (8.5 kDa); that is, monoubiquitylation.
In addition, when nascent adhesions assemble, there is a continuous increase in intensity consistent with addition of paxillin monomers, whereas when the adhesions dissemble, the loss of intensity occurs in larger steps compatible with release of larger aggregates (E. Gratton and M. Digman, unpublished observation).
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