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As mentioned above, for consistency to previous work on cold acclimation and the effect of cold on the circadian clock, we used 16 h photoperiod and soil grown plants.
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For consistency to our previous works, we normalize the sum per vector and per dimension.
It describes an automated methodology used to measure segregation and evaluates that method for consistency, repeatability, and correlation to previous methods.
The Cronbach's alpha coefficient of 0.93 indicated a good internal consistency and is similar to previous reported values.
The consistency of the present findings to previous qualitative research points to the face validity and appropriateness of our scoring system.
The hylan G-F 20 used in this study was diluted to 3.3 mg/mL from its clinical concentration of 8 mg/mL to provide consistency with previous studies characterizing the PRG4 + HA interaction and investigate the effect of cross-linking.
The tolerance for tempo detection hereby is 3.5% relative BPM deviation to maintain consistency with previous publications [32].
In order to maintain consistency with previous work on compounds 1 – 3 [1], we have used the original 2,4,6 numbering of the phosphorus atoms for the cyclophosphazene ring rather than change to the 1,3,5 numbering using IUPAC convention.
In our hands staining pDC with CD45RA faithfully recapitulates staining with more recently developed antibodies such as pPDCA-1, therefore CD45RA was used to maintain consistency with previous studies.
However, these search terms were used to maintain consistency with previous research [ 2, 12].
To maintain consistency with previous studies, cultures treated with 1 mmol/L LiCl were incubated for 72 hours as previously described (39) before harvest for dual luciferase assay.
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