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Multiple protein alignments were constructed using the MUSCLE program [ 54] with default parameters and manually checked for errors and for consistency of the alignment with the ScanProsite [ 51] ICL and MS amino acid patterns.
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The Enredo and Pecan (which together with Ortheus) comprise the EPO pipe line assures the consistency of the alignments with paralogs [ 53].
Consistency: We first measured the consistency of the predicted network alignment by computing the mean entropy of the predicted clusters.
We conclude that there is substantial scope for improving the consistency of structural alignment methods.
Criteria applied manually to judge candidate members of the PEP-CTERM family for inclusion in new rounds of model building included overall sequence similarity to other members, conserved position near the protein C-terminus, quality of the predicted transmembrane helix and other motifs, and consistency of the resulting multiple sequence alignment.
A third researcher performed spot-checks to ensure the consistency of themes and alignment with the study aims.
We find the most common partner for each tissue-selective gene and use a consensus rate, represented by τ, to summarize each rows of the alignment consistency table.
These anchors are calculated using a greedy depth-first search of the alignment graph ordered by consistency score, traversing the highest consistency edges first (Fig. 2).
Detailed studies of traffic safety, as highway design consistency evaluation, require having the geometric definition of the alignment.
The alignment was then manually adjusted to ensure consistency with pfam00379, particularly at the edges of the alignment, and to ensure that alignments among sets of highly similar genes were not distorted by the global optimum.
The most striking aspect of the results presented here is the positional consistency of the similarities, in terms of identified alignments, between all the test databases – indicating that it is not just a consequence of random differences in sampling between the databases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com