The GCI was established according to the intrinsic features of "ghost cities", comprising three criteria: consistency of lit area and built-up area, illumination intensity and population density.
The maps of other layers also suggest the consistency of the area borders determined from the layer 4 map (Fig. 5D).
On the basis of this sequence, we designed the PCR primers (see Supplementary Table S1), and the consistency of the area difference was confirmed between 2 individuals by semiquantitative RT-PCR.
As the location of maximum activation may also vary between sessions (i.e. ON/OFF), a dispersion of coordinates up to 4 mm, equivalent to the radius of the extracted sphere, was allowed between sessions to ensure within-subject consistency of anatomical areas in patients.
The simultaneous inspection of the Manhattan plots for the two populations suggested some consistency of genome areas hosting putative QTLs, such as those around the end of the chromosomes 1 and 6, or an area in the last third of chromosome 8 (Fig. 6).
Then, the two virtual images are merged together to reduce the hole regions and maintain the temporal consistency of these areas.
To this end a check list of potential research topics has been developed to enable us to retain some consistency of objectives from area to area.
Substituting this value for calculations of areas and circumferences of circles with diameters 1, 2, … up to 9, he obtained numbers which showed consistency of circumference and area, 'thereby furnishing incontrovertible proof of the exact truth' of his ratio.
The consistency of cross-sectional area measurements obtained from OPT with those obtained by planimetric analysis of the corresponding histologically-stained sections (the current gold standard) confirmed the accuracy of the former.
Yet it was in exploring these challenges that we came to the conclusion that life course area deprivation is best represented by the percentage of all occupied persons in an area with a low social class occupation; as it was the only area measure to have sufficient variability across areas, consistency of meaning, and to be consistently related to other area measures at all study years.
