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Size distribution analysis showed that both the matched and unmatched categories predominantly consisted of reads approximately 22 23 nt in length (Fig. 1b).
These consisted of reads that we tentatively identify as belonging to 21U, 22G, and 26G classes.
Set #3 consisted of reads enriched in full-length (FL) cDNAs.
The second peak occurred at 30 33 nt and consisted of reads mapping to tRNA genes.
Therefore, informative reads consisted of reads representing the transgene as well as reads representing the endogenous mouse Prnp locus (Additional file 3).
Normalized results, which consisted of reads per kilobase of exon per million mapped reads (RPKM) values, were used to represent gene expressions.
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