Considering FISH as the gold standard technique, CISH demonstrated a great specificity but a low sensibility, since just 37.5% of amplified FISH cases were detected by CISH.
To avoid considering FISH artifacts as false-positive chromosome losses or gains, we have introduced two independent approaches: QFISH and interphase chromosome-specific MCB, allowing accurate definition of the normal thresholds of aneuploidy in human somatic cells [15], [19].
While there may be advantages in considering fish densities at each station rather than their presence or absence alone, the present study encompassed a wide geographic region.
Initially, the tendency was to assume that either risk or benefit was the most important parameter when considering fish consumption advisories.
Considering FISH as gold standard, the sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) were calculated for RT-PCR technique.
The predictive capabilities of each experimental technique were described by concordance percentage, sensitivity, specificity, and positive and negative predictive values, considering FISH as the reference technique.
