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Adapting from Petersen's criteria [42] presented earlier (chapter 2.1), a more simplified version of semi-quantitative (QUAN) and qualitative (QUAL) implications was considered to score the black swans.
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Scores <1.7 were considered to unreliable, scores ≥1.7 × <2 were considered to be reliable to the genus level; however, if database match number 1 and 2 resulted in the same strain name, it was considered correct to the species level, and scores ≥2 were considered to be reliable to the species level.
Scores that have Specificity p-values less than 0.05 are considered to be scores that can be attributed with high confidence to the HYP of interest.
Of the various models suggested, structures which had a higher C-score were taken (C-score considered to be a confidence score for estimating the quality of predicted models by I-TASSER).
An abnormal outcome on each subscale is a score < 70, and those at risk are considered to have a score of between 70 and 85.
In cases where differences between duplicate tissue cores were observed, the higher score was considered to be the final score.
Any nucleotide with a redundant International Union of Pure and Applied Chemistry code was considered to have a score of zero.
Scores 2 and 3 were considered to indicate overexpression, scores 0 and 1 were considered as negative.
Participants situated above the 75th percentile (P75) for each dimension of the BCSQ-12-SS were considered to have "high scores", whereas those situated below the 75th percentile were considered to have "low scores" [ 17, 25, 26].
Hybridisation signals from other cell types (e.g. lymphocytes) were not considered to avoid possible scoring of napsin B expression.
Briefly, the Pygo2 staining intensity was scored as − (no signal), + (weak), ++ (moderate), +++ (strong); to scores were considered to be negative (normal Pygo2 expression) and to scores were considered to be positive (abnormal Pygo2 expression).
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